Ovarian PRP for Diminished Ovarian Reserve

(oPRP Trial)

Overseen By Jesse Hade, MD

Age: 18 - 65

Sex: Female

Trial Phase: Academic

Sponsor: Generation Next Fertility

Disqualifiers: Premature ovarian failure, Menopause, others

Compensation: Varies

Number of Visits: 2

Duration: 12 months

230 Participants Needed

What You Need to Know Before You Apply

What is the purpose of this trial?

This trial explores whether injections of ovarian platelet-rich plasma (oPRP) can improve IVF outcomes for women with diminished ovarian reserve (DOR). DOR refers to having fewer eggs in the ovaries than expected for someone of the same age, making pregnancy more challenging. Participants will receive either oPRP injections or a placebo (an inactive substance) to compare results. Women diagnosed with DOR and experiencing poor ovarian response (limited egg production) may be suitable candidates. As an unphased trial, this study offers participants the chance to contribute to groundbreaking research that could enhance fertility treatments.

Will I have to stop taking my current medications?

The trial information does not specify whether you need to stop taking your current medications. It's best to discuss this with the trial coordinators or your doctor.

What prior data suggests that ovarian PRP injections are safe for women with diminished ovarian reserve?

Research shows that ovarian platelet-rich plasma (oPRP) treatments may improve fertility in women with diminished ovarian reserve (DOR). Studies have found that oPRP injections can enhance ovarian function and increase egg production. Specifically, one study noted a significant improvement in fertility for women with DOR after receiving PRP treatment.

Early clinical data also suggest that women who received oPRP injections directly into the ovaries had better chances of becoming pregnant. This indicates that the treatment might improve pregnancy chances for those with low ovarian reserves.

Regarding safety, these studies indicate that oPRP is generally well-tolerated, with no major reports of serious side effects. This is encouraging for those considering the treatment. However, as with any medical procedure, discussing potential risks and benefits with a healthcare provider is important. ^{1 2 3 4 5}

Why are researchers excited about this trial?

Most treatments for diminished ovarian reserve involve hormone therapies that aim to stimulate the ovaries. However, oPRP is unique because it harnesses the patient’s own platelet-rich plasma (PRP) and injects it directly into the ovaries. This approach could potentially rejuvenate ovarian tissue and improve egg quality by using the body’s natural growth factors. Researchers are excited about this treatment because it offers a personalized and potentially less invasive alternative to traditional hormone treatments, with the promise of enhancing fertility outcomes.

What evidence suggests that oPRP might be an effective treatment for diminished ovarian reserve?

Research shows that ovarian platelet-rich plasma (oPRP) injections, which participants in this trial may receive, can help women with low ovarian reserves increase their chances of getting pregnant. Studies have found that oPRP treatment improves ovarian function and leads to more pregnancies and live births. For example, one study found that about 16.6% of women had a live birth after PRP treatment. Another analysis showed that about 21% of women became pregnant after the treatment. These findings suggest that oPRP could be a promising option for women with low ovarian reserves. ^{4 6 7 8 9}

Who Is on the Research Team?

Jesse Hade, MD

Principal Investigator

Generation Next Fertility

Are You a Good Fit for This Trial?

This trial is for women aged 35-42 with a clinical diagnosis of diminished ovarian reserve or poor ovarian response, which typically results in lower pregnancy and live birth rates.

Inclusion Criteria

I have been diagnosed with low ovarian reserve.

I am a woman aged between 35 and 42.

I have been diagnosed with poor ovarian response.

Exclusion Criteria

I have been diagnosed with early menopause.

I have been clinically diagnosed with menopause.

I am under 35 years old.

Timeline for a Trial Participant

Screening

Participants are screened for eligibility to participate in the trial

2-4 weeks

1 visit (in-person)

Baseline Assessment

Baseline ultrasound and blood work at the time of menses, approximately one month prior to starting IVF cycle

4 weeks

1 visit (in-person)

Treatment

Participants receive ovarian injections of either oPRP or placebo, followed by IVF cycle with monitoring and oocyte retrieval

12 months

Multiple visits (in-person) for monitoring and procedures

Follow-up

Participants are monitored for safety and effectiveness after treatment, including assessment of ovarian response and embryo availability

12 months

What Are the Treatments Tested in This Trial?

Interventions

• oPRP

Trial Overview The study is testing if injecting platelet-rich plasma (oPRP) into the ovaries can improve IVF outcomes compared to a placebo. Women are randomly assigned to receive either oPRP or a serum without active ingredients.

How Is the Trial Designed?

Group I: oPRP injections Experimental Treatment 1 Intervention

Patients own prepared PRP will be injected into each ovary

Group II: Placebo Placebo Group 2 Interventions

Patient's serum will be injected into the ovary (not rich with platelets)

Who Is Running the Clinical Trial?

Generation Next Fertility

Lead Sponsor

Trials

1

Recruited

230+

Igenomix

Industry Sponsor

Trials

38

Recruited

16,800+

Published Research Related to This Trial

Intra-ovarian injection of platelet-rich plasma (PRP) significantly increased anti-Müllerian hormone (AMH) levels in 22 women with poor ovarian reserve, suggesting a potential improvement in fertility.

While AMH levels rose after PRP treatment, there were no significant changes in antral follicle counts (AFC), indicating that while PRP may enhance ovarian function, it does not necessarily increase the number of visible follicles.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Intra-ovarian infusion of autologous platelet-rich plasma in women with poor ovarian reserve: A before and after study. Hosseinisadat, R., Farsi Nejad, A., Mohammadi, F.[2022]

Intraovarian injection of platelet rich plasma (PRP) shows promise in improving ovarian reserve markers and increasing oocyte and embryo yields in patients with poor ovarian response (POR) and primary ovarian insufficiency (POI), as indicated by several case series and one prospective trial.

Patients who previously failed treatment have reported multiple live births after receiving PRP injections, suggesting that this novel treatment could enhance fertility outcomes for those with poor prognosis.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

The use of intraovarian injection of autologous platelet rich plasma (PRP) in patients with poor ovarian response and premature ovarian insufficiency. Herlihy, NS., Seli, E.[2022]

In a clinical trial involving 35 women with poor ovarian reserve, a single intraovarian injection of autologous platelet-rich plasma (PRP) significantly increased the number of oocytes and embryos produced, indicating its potential efficacy in improving ovarian response.

The treatment also led to elevated estradiol levels, suggesting that PRP may enhance ovarian function, although more research is needed to determine its impact on live birth rates.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Intraovarian Injection of Autologous Platelet-Rich Plasma Improves Therapeutic Approaches in The Patients with Poor Ovarian Response: A Before-After Study. Navali, N., Sadeghi, L., Farzadi, L., et al.[2022]

In a study of 80 women aged 28-54 with extremely low functional ovarian reserve, intraovarian injection of platelet-rich plasma (PRP) did not show any clinically significant improvements in ovarian function over a 1-year follow-up period.

Despite the lack of overall efficacy, two patients achieved pregnancy after PRP treatment, suggesting that while sporadic pregnancies are possible in this population, the study does not confirm that PRP was the cause of these pregnancies.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Preliminary report of intraovarian injections of autologous platelet-rich plasma (PRP) in extremely poor prognosis patients with only oocyte donation as alternative: a prospective cohort study. Barad, DH., Albertini, DF., Molinari, E., et al.[2022]

In a study of 96 women with poor ovarian response (POR), intra-ovarian administration of platelet-rich plasma (PRP) significantly increased the total number of oocytes and the number of mature (MII) oocytes across most groups, indicating its potential efficacy in enhancing ovarian function.

Despite the increase in oocyte numbers, changes in hormone levels (LH, FSH, AMH, and estradiol) were not significant, and only 14.6% of participants achieved clinical pregnancy, suggesting that while PRP may improve oocyte yield, its impact on overall fertility outcomes needs further investigation.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Evaluation of intra-ovarian platelet-rich plasma administration on oocytes-dependent variables in patients with poor ovarian response: A retrospective study according to the POSEIDON criteria. Farimani, M., Nazari, A., Mohammadi, S., et al.[2022]

Platelet-rich plasma (PRP) therapy may improve ovarian function and increase pregnancy chances in women with very low ovarian reserve and premature ovarian insufficiency, based on a review of 25 studies and 2 abstracts.

Despite some skepticism about PRP's role in fertility treatments, its low-risk profile and potential benefits warrant further investigation through larger randomized controlled trials.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Ovarian response to intraovarian platelet-rich plasma (PRP) administration: hypotheses and potential mechanisms of action. Seckin, S., Ramadan, H., Mouanness, M., et al.[2023]

In a study involving 38 women aged 31-45 with low ovarian reserves, PRP (platelet-rich plasma) injections into the ovaries led to significant improvements in hormone levels and resulted in ten pregnancies, including six healthy births, demonstrating the efficacy of this treatment for premature ovarian insufficiency.

The study introduced a novel laparoscopically assisted technique for administering PRP, achieving a high platelet concentration that contributed to long-lasting results, with benefits observed up to 12 months after a single procedure.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Ovarian Rejuvenation Through Platelet-Rich Autologous Plasma (PRP)-a Chance to Have a Baby Without Donor Eggs, Improving the Life Quality of Women Suffering from Early Menopause Without Synthetic Hormonal Treatment. Petryk, N., Petryk, M.[2020]

In a pilot study involving 4 women with diminished ovarian reserve, the injection of autologous platelet-rich plasma (PRP) into the ovaries led to improved ovarian function, as indicated by increased serum AMH levels and decreased FSH levels, allowing for the retrieval of viable oocytes for IVF.

This is the first report of direct PRP injection into human ovaries for infertility treatment, showing promising results in enhancing ovarian reserve and function, although further research is needed to understand the specific components of PRP that contribute to these effects.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

First data on in vitro fertilization and blastocyst formation after intraovarian injection of calcium gluconate-activated autologous platelet rich plasma. Sills, ES., Rickers, NS., Li, X., et al.[2022]

In a study involving 20 poor ovarian responder (POR) women, intra-ovarian injection of platelet-rich plasma (PRP) was found to be safe and showed a tendency to improve ovarian reserve markers, including anti-Mullerian hormone (AMH) and estradiol levels, as well as the number and quality of oocytes.

Despite these improvements, the changes were not statistically significant, and the overall pregnancy rates remained low, with only 15% achieving chemical pregnancy and 5% achieving clinical pregnancy.

NCBI (Pubmed)

pubmed.ncbi.nlm.nih.gov

Effect of intra-ovarian platelet rich plasma in women with poor ovarian response. Davari Tanha, F., Salimi Setudeh, S., Ebrahimi, M., et al.[2023]

Citations

1. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov/articles/PMC11100055/

Platelet-rich plasma (PRP) treatment of the ovaries ... PRP treatment resulted in a statistically significant improvement in the main fertility parameters of diminished ovarian reserve women.

2. sciencedirect.com sciencedirect.com/science/article/pii/S2352320425001907

Research trends on platelet-rich plasma in improving ... The summary results of the Meta-Analysis indicated that injection of intraovarian PRP could promote ovarian regeneration and improve the reproductive outcomes ...

3. nature.com nature.com/articles/s41598-025-02689-2

Comparative efficacy of single vs. double autologous ... This study aims to compare the efficacy of single versus double PRP ovarian injections in improving ovarian reserve, response to stimulation, and IVF/ICSI ...

4. pubmed.ncbi.nlm.nih.gov pubmed.ncbi.nlm.nih.gov/38214306/

The efficacy of platelet rich plasma on women with poor ... Results: A total of 10 trials were enrolled in the present meta-analysis. Following PRP treatment, live birth rate was found to be 16.6% (95% CI 8.8%-26.1%), ...

5. eurjmedres.biomedcentral.com eurjmedres.biomedcentral.com/articles/10.1186/s40001-025-02854-3

Evaluation of intraovarian injection of platelet-rich plasma for ... The meta-analysis revealed a pregnancy ratio of 0.21 and a live birth ratio of 0.18 in women with poor ovarian reserve after PRP injection.

6. pmc.ncbi.nlm.nih.gov pmc.ncbi.nlm.nih.gov/articles/PMC12109040/

Platelet-Rich Plasma (PRP) in Reproductive Medicine PRP has the potential to stimulate follicular growth, improving ovarian function and increasing egg production, even in cases of diminished ...

7. sciencedirect.com sciencedirect.com/science/article/abs/pii/S016503782500227X

The impact of platelet-rich plasma (PRP) on ovarian ... Early clinical data show better reproductive outcomes post-intraovarian PRP in women with low ovarian reserve or POI. •. Validating PRP's role in fertility ...

8. ovarianresearch.biomedcentral.com ovarianresearch.biomedcentral.com/articles/10.1186/s13048-024-01423-2

Platelet-rich plasma (PRP) treatment of the ovaries ... PRP treatment resulted in a statistically significant improvement in the main fertility parameters of diminished ovarian reserve women.

9. academic.oup.com academic.oup.com/humrep/article-abstract/doi/10.1093/humrep/deaf097.1097/8170537

P-793 Ovarian rejuvenation in poor responders with plasma ... Ovarian PRP could improve fertility outcomes and can be a potential option for women with poor ovarian reserve.