Resistance Exercise for Muscle-Fat Communication

(TIMER2 Trial)

YW
Overseen ByYuan Wen, MD/PhD
No Placebo GroupAll trial participants will receive the active study treatment (no placebo)

What You Need to Know Before You Apply

What is the purpose of this trial?

This trial explores how exercise influences communication between muscle and fat cells through tiny packages called extracellular vesicles (EVs). These vesicles carry important molecules that may alter how the body processes sugar and fat. Participants will perform resistance exercises, such as squats and leg presses, to help researchers understand this cell communication. Individuals with a sedentary lifestyle (exercising less than once a week) and either normal glucose levels or prediabetes may be suitable for this trial. As an unphased trial, this study offers a unique opportunity to contribute to groundbreaking research on exercise and cell communication.

Will I have to stop taking my current medications?

The trial excludes people who use medications that significantly affect glucose metabolism or exercise response, so you may need to stop taking such medications to participate.

What prior data suggests that this exercise protocol is safe?

Research shows that resistance exercise is generally safe for most people. Studies have found that this type of exercise can reduce body fat and increase strength without causing major problems. When performed correctly, exercises like squats and leg presses have not led to serious safety issues. Using the right form and following instructions are crucial to prevent injuries. Overall, resistance exercise is well-tolerated and can safely improve health and fitness.1234

Why are researchers excited about this trial?

Researchers are excited about this trial because it explores how resistance exercise might change the way muscles and fat communicate in the body. Unlike traditional treatments for improving muscle and fat health, which often involve medication or dietary changes, this approach uses acute resistance exercises like squats, knee extensions, leg presses, and lat pulldowns. These exercises operate at 80% of a person's maximum capacity, potentially offering a more dynamic and engaging way to enhance muscle and fat interactions. By understanding these interactions better, scientists hope to develop new strategies for improving metabolic health without relying solely on pharmaceuticals.

What evidence suggests that acute resistance exercise is effective for muscle-fat communication?

Research has shown that resistance exercise, such as the Acute Resistance Exercise in this study, helps reduce body fat and improve body shape. One study found a noticeable drop in body fat percentage, with a change of −2.2%. Another study discovered that high-intensity resistance training led to more fat loss than low-intensity training. Resistance training also reduces fat in specific areas, like the belly, which is important for addressing obesity. These findings suggest that resistance exercise can effectively improve how the body handles sugar and fat.56789

Who Is on the Research Team?

YW

Yuan Wen, MD, PhD

Principal Investigator

University of Kentucky

Are You a Good Fit for This Trial?

This trial is for people aged 30-55 who are mostly inactive, can consent to participate, and fit into two groups: a control group with BMI under 27, normal blood sugar, and at most one metabolic syndrome feature; or a prediabetic group with BMI over 30, prediabetes, and at least three metabolic syndrome features.

Inclusion Criteria

I am between 30 and 55 years old.
I exercise less than once a week.
I can understand and agree to the study's procedures and risks.
See 2 more

Timeline for a Trial Participant

Screening

Participants are screened for eligibility to participate in the trial

2-4 weeks
1 visit (in-person)

Baseline Assessment

Baseline blood draw and tissue biopsies are conducted one hour prior to exercise

1 day
1 visit (in-person)

Treatment

Participants undergo whole-body resistance training at 80% 1RM intensity, followed by post-exercise blood samples and biopsies

1 day
1 visit (in-person)

Laboratory Analysis

Laboratory analyses include miR-1 quantification, EV uptake assessment, RNA sequencing, and EV isolation

1-2 weeks

Follow-up

Participants are monitored for safety and effectiveness after treatment

4 weeks

What Are the Treatments Tested in This Trial?

Interventions

  • Acute Resistance Exercise

Trial Overview

The study investigates how muscle cells signal fat cells using extracellular vesicles during resistance exercise. It focuses on the role of microRNA-1 in these communications by comparing subjects with high versus low changes in miR-1 after exercising.

How Is the Trial Designed?

1

Treatment groups

Experimental Treatment

Group I: Acute Resistance ExerciseExperimental Treatment1 Intervention

Find a Clinic Near You

Who Is Running the Clinical Trial?

Yuan Wen

Lead Sponsor

Trials
1
Recruited
40+

University of Kentucky

Lead Sponsor

Trials
198
Recruited
224,000+

National Institute of General Medical Sciences (NIGMS)

Collaborator

Trials
315
Recruited
251,000+

Citations

Resistance training effectiveness on body composition and ...

Resistance‐based exercise programs resulted in significant reductions in body fat percentage (number of studies [k] = 89, ES = −2.2%, 95% CI: −2.4 to −2.0%) and ...

Effects of resistance training and aerobic ...

This study demonstrated that resistance training effectively reduced fat content in the Android region and improved abdominal obesity.

Impact of resistance training intensity on body composition ...

Results: Post-test results showed a significant reduction in body fat percentage for the high-intensity group compared with the low-intensity (p = 0.035) and ...

Comparison of concurrent, resistance, or aerobic training ...

CT reduced significantly more fat mass compared to RT (MD: −1.09 kg [95% CI = -0.27 to −1.91]; p = 0.009). No significant differences were found between CT, AT, ...

Effect of Resistance Exercise on Body Composition and ...

This systematic review aimed to summarise the existing evidence on the effectiveness of resistance exercise on body composition, functional capacity, and muscle ...

Associations of muscle-strengthening exercise with ...

Besides, one study showed that aerobic training intervention had small effects on reducing body fat and body mass percentage among adolescents (Schranz et al., ...

State Operations Manual Appendix PP

... (fat) is not visible and deeper tissues are not visible. Granulation tissue, slough and eschar are not present. This stage should not be used to describe ...

Strength: Systematic Review And Meta-Analysis Master List

... lifting first or cardio first) didn't affect gains in aerobic fitness, changes in body fat percentage, or lower body isometric strength, but it did affect ...

How Senomorphic Therapy Could Reverse Aging Muscle ...

Early studies using combinations such as dasatinib and quercetin showed promise in clearing senescent cells in tissues like lung and fat ...