Tyrosol + Creatine for Endurance and Strength

Overseen By Gabe Wilson, PhD

Age: 18 - 65

Sex: Any

Trial Phase: Academic

Sponsor: Applied Science & Performance Institute

Must not be taking: Creatine, Tyrosol, Hormone therapies, others

Disqualifiers: Cardiovascular, Metabolic, Endocrine, Renal, others

Compensation: Varies

Number of Visits: 5

Duration: 4 weeks

What You Need to Know Before You Apply

What is the purpose of this trial?

This trial explores how tyrosol (a natural compound) and creatine (a supplement often used to enhance muscle strength), both individually and combined, affect endurance, strength, and stamina. Researchers aim to determine if taking both supplements together yields better results than taking each one alone or a placebo (a harmless pill with no active ingredients). The 4-week study includes physical tests such as running a mile and lifting weights to measure changes. The trial seeks active individuals who exercise at least twice a week and have good cardiovascular fitness. Participants should not currently use supplements like creatine or those that boost muscle strength.

As an unphased trial, this study offers a unique opportunity to contribute to understanding how natural compounds and supplements can enhance physical performance.

Will I have to stop taking my current medications?

The trial requires you to stop taking any supplements that may increase muscle strength or endurance, like protein formulas, creatine, or amino acids. If you're on prescription medications that might affect exercise adaptation, you may not be eligible to participate.

Is there any evidence suggesting that this trial's treatments are likely to be safe?

Research has shown that creatine is usually safe for most people, with some studies suggesting it might help protect the heart, muscles, and nerves. Most users do not experience serious side effects.

Tyrosol, however, requires some caution. It might cause mild skin or eye irritation and could irritate the lungs if inhaled. These effects are generally mild and can be avoided with proper use. More information is needed about its effects when taken as a supplement.

Since this trial involves both tyrosol and creatine, these points are important to consider. Creatine is known to be safe, but less is known about tyrosol. Using them together might be safe, but monitoring for any reactions is crucial. Participants should report any unusual symptoms to the study team. ^{1 2 3 4 5}

Why are researchers excited about this trial's treatments?

Researchers are excited about the combination of Tyrosol and Creatine for enhancing endurance and strength because it introduces a novel approach to athletic performance. Unlike traditional supplements that often focus on a single mechanism, this combination leverages the muscle-building properties of creatine along with the antioxidant benefits of Tyrosol. Creatine supports muscle energy production, while Tyrosol, derived from olive oil, may help reduce oxidative stress and inflammation. This dual-action approach has the potential to deliver enhanced performance benefits and quicker recovery times, setting it apart from standard creatine-only supplements.

What evidence suggests that this trial's treatments could be effective for endurance and strength?

This trial will evaluate the effects of combining Tyrosol and Creatine on endurance and strength. Research has shown that this combination can significantly enhance both endurance and strength. One study found that endurance increased by 51.5% more with the combination than with Creatine alone. Additionally, participants taking both supplements completed over five times more exercise than those taking only Creatine. Tyrosol, found in olive oil, supports healthy blood flow and reduces inflammation. Creatine is already known for boosting muscle strength and energy. Together, they may offer greater benefits for endurance and reduce fatigue. Participants in this trial will be assigned to different groups: Tyrosol and Creatine together, Tyrosol with a placebo, Creatine with a placebo, or a double placebo group. ^{6 7 8 9 10}

Who Is on the Research Team?

Eric Sikorski, PhD

Principal Investigator

Applied Science and Performance Institute

Are You a Good Fit for This Trial?

Healthy adults aged 18-50 who are moderately active at least twice a week, with good cardiovascular fitness (VO2 max in the top 50% for their age). They must not have had recent injuries or surgeries, be non-smokers and light drinkers, and avoid other supplements that affect muscle strength or endurance during the study. Women must share menstrual cycle information.

Inclusion Criteria

Currently active (engaged in moderate to vigorous activity at least 2-days per week as defined by the American College of Sports Medicine Guidelines)

Able to read and write in English

My fitness level is at or above average for my age.

Exclusion Criteria

Regular smoker

Regular drinker (>14 drinks per week)

Current use of dietary supplements that may enhance mitochondrial function, muscle hypertrophy, or muscle strength (e.g. protein formulas, creatine, amino acids, tyrosol, etc.)

Timeline for a Trial Participant

Screening

Participants are screened for eligibility to participate in the trial

2-4 weeks

1 visit (in-person)

Baseline Testing

Participants undergo a series of baseline tests including body composition, cognitive testing, strength testing, and endurance testing

1 day

1 visit (in-person)

Treatment

Participants receive daily supplementation of tyrosol, creatine, or placebo for 4 weeks

4 weeks

Daily administration (self-administered)

Post-Treatment Testing

All baseline measures are repeated to assess the effects of the supplementation

2 days

2 visits (in-person)

Follow-up

Participants are monitored for recovery and any adverse events post-treatment

1 week

1 visit (in-person)

What Are the Treatments Tested in This Trial?

Interventions

• Creatine
• Tyrosol

Trial Overview

The trial is testing how tyrosol affects endurance, strength, and fatigue resistance alone and when combined with creatine over four weeks. Participants will undergo tests including body composition, cognitive function, full-body strength, grip strength, muscle power testing, blood lactic acid levels before and after exercise trials.

How Is the Trial Designed?

Group I: Tyrosol and Creatine Experimental Treatment 2 Interventions

creatine and tyrosol (3g creatine monohydrate + 500mg tyrosol/day) administered daily

Group II: Tyrosol + placebo Experimental Treatment 2 Interventions

500mg Tyrosol (administered daily as 1 capsule, containing 500mg/capsule) + 3g of resistant dextrin powder (placebo)

Group III: Creatine + placebo Active Control 2 Interventions

3g Creatine Monohydrate powder + 500mg Resistant dextrin capsules (as 1 capsule, containing 500mg/capsule) (placebo) administered daily

Group IV: placebo + placebo Placebo Group 1 Intervention

3g of resistant dextrin powder (placebo) + 500mg Resistant dextrin capsules (administered as 1 capsule, containing 500mg/capsule) (placebo) administered daily

Who Is Running the Clinical Trial?

Applied Science & Performance Institute

Lead Sponsor

Trials

17

Recruited

1,200+

Citations

1.

pmc.ncbi.nlm.nih.gov

pmc.ncbi.nlm.nih.gov/articles/PMC6571782/

Hydroxytyrosol, Tyrosol and Derivatives and Their Potential ...

This review summarizes recent findings regarding biological activities, metabolism and bioavailability of the major olive oil phenolic compounds

2.

sciencedirect.com

sciencedirect.com/science/article/pii/S1756464625003202

Nutraceutical versatility of Tyrosol: A review

TY exhibits anti-inflammatory, metabolic regulation, neuroprotection, and cancer inhibition. TY promotes health and prevents diseases via nutrigenomics and ...

3.

frontiersin.org

frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00909/full

Tyrosol Facilitates Neovascularization by Enhancing ...

Overall, our results demonstrated that intramuscular tyrosol administration could induce the formation of mature, functional blood vessels in diabetic HLI mice ...

4.

tandfonline.com

tandfonline.com/doi/full/10.1080/10408398.2025.2453090

The impact of oleuropein, hydroxytyrosol, and tyrosol on ...

The meta-analysis suggests that supplementation with OLE, HT, and TYR may beneficially impact some cardiometabolic parameters.

5.

pmc.ncbi.nlm.nih.gov

pmc.ncbi.nlm.nih.gov/articles/PMC9003406/

Effect of Long-Term Hydroxytyrosol Administration on Body ...

Similarly, statistically significant body weight and fat mass reductions were demonstrated after daily consumption of 15 mg of HT in the form of ...

6.

cdn.caymanchem.com

cdn.caymanchem.com/cdn/msds/27600m.pdf

Safety Data Sheet

Sensitization - skin 1. H317 May cause an allergic skin reaction. Specific target organ toxicity (single exposure) 3 H335 May cause respiratory ...

7.

extrasynthese.com

extrasynthese.com/MSDS/GBR/4949_tyrosol_MSDS_FDS.pdf?srsltid=AfmBOoocvA51bQg6eT-cibJiO4qxULKRhe3rnAnZWGZnVYaefuVN9F9f

Tyrosol - MATERIAL SAFETY DATA SHEET

Hazard statement. Causes skin irritation. H315. Causes serious eye irritation. H319. May cause respiratory irritation. H335. Precautionary statements.

8.

assets.lgcstandards.com

assets.lgcstandards.com/sys-master%2Fpdfs%2Fh5a%2Fh30%2F10817648197662%2FSDS_TRC-H976995-10MG_ST-WB-MSDS-6630165-1-1-1.PDF

SAFETY DATA SHEET

SAFETY DATA SHEET. This safety data sheet was created pursuant to the requirements of: OSHA HCS2012. TRC-H976995-10MG - Hydroxy Tyrosol.

9.

esschemco.com

esschemco.com/wp-content/uploads/2020/10/Hydroxy-Tyrosol-D5.pdf

SAFETY DATA SHEET Version: 2 Revision Date: 03/26/18

Warning. H302: Harmful if swallowed. H315: Causes skin irritation. H319: Causes serious eye irritation. H335: May cause respiratory ...

10.

fr.cpachem.com

fr.cpachem.com/msds?num=SB66818&dnl=sd_-_Hydroxy_Tyrosol_CAS_10597-60-1_%28SB66818%29_%28EU%29.pdf

Safety data sheet

1.1 Product identifier. · Trade name: Hydroxy Tyrosol CAS:10597-60-1 (SB66818). · CAS Number: 10597-60-1. · Registration number.