Measurement of Water in Breath and Saliva for Human Energy Expenditure
What You Need to Know Before You Apply
What is the purpose of this trial?
This trial explores a new method to measure certain elements in breath and saliva to better understand how the body uses energy. Researchers aim to track these elements without administering any special doses to participants. The study seeks healthy adults living in the Denver area, specifically those without major illnesses like diabetes or heart problems and who are not obese. Participants should identify as healthy and be between 18 and 45 years old. As an unphased trial, this study allows participants to contribute to groundbreaking research in energy metabolism without the risks associated with new treatments.
Will I have to stop taking my current medications?
The trial information does not specify whether you need to stop taking your current medications. However, since the study involves healthy adults without chronic diseases, it might be best to discuss your medications with the trial team.
What prior data suggests that this method is safe for measuring isotopes in saliva and breath?
Research has shown that deuterium and oxygen-18 are stable forms of elements, meaning they do not break down or emit radiation. Studies have found these forms safe for tracking in human samples like saliva and urine over time.
Further research supports using these forms to measure body water. Deuterium, often administered as deuterium oxide, mixes with the body's water without causing harm.
Overall, these forms have a well-documented safety record in humans. They are used in many studies without reports of negative effects, indicating they are well-tolerated for measurement purposes in studies like this one.12345Why are researchers excited about this trial?
Researchers are excited about this trial because it explores a new method of measuring human energy expenditure by analyzing the isotopes 2H:1H and 18O:16O in saliva and breath. Unlike traditional methods that often require more invasive procedures or equipment to estimate energy use, this approach could offer a non-invasive and easily repeatable alternative. By focusing on healthy adults, the trial aims to refine the accuracy of this technique, potentially paving the way for more personalized and accessible health monitoring.
What evidence suggests that this method is effective for measuring human energy expenditure?
Research shows that measuring certain types of water in saliva and breath reveals how much energy a person uses. These special types of water, called isotopes, can also be measured in other body fluids like urine. This accurate method helps researchers learn about a person's energy use over time. It employs a technique known as the doubly labeled water method, which tracks how these isotopes move through the body. Although still under study, early results suggest it could reliably assess a person's energy needs. Participants in this trial will specifically focus on the feasibility of measuring these isotopes in saliva and breath.678910
Who Is on the Research Team?
Edward L Melanson, PhD
Principal Investigator
University of Colorado, Denver
Are You a Good Fit for This Trial?
This trial is for healthy men and women aged 18-45 who are not pregnant or breastfeeding, do not have acute or chronic diseases like diabetes, heart or thyroid conditions, and are not obese (BMI > 30 kg/m2).Inclusion Criteria
Exclusion Criteria
Timeline for a Trial Participant
Screening
Participants are screened for eligibility to participate in the trial
Baseline Measurement
Evaluation of the natural abundances of 2H:1H and 18O:16O in urine, saliva, and breath of humans
Follow-up
Participants are monitored for any changes in isotopic measurements post-baseline
What Are the Treatments Tested in This Trial?
Interventions
- Feasibility of measuring 2H:1H and 18O:16O in saliva and breath
Trial Overview
The study is testing the feasibility of measuring natural abundances of hydrogen and oxygen isotopes in saliva and breath to understand human energy expenditure better.
How Is the Trial Designed?
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Treatment groups
Experimental Treatment
Healthy adults (18-45 yrs.) will be recruited from the local Denver area. The investigators have elected to study a relatively homogenous sample to limit the impact of age, weight, and chronic disease on isotope fractionation in breath.
Find a Clinic Near You
Who Is Running the Clinical Trial?
University of Colorado, Denver
Lead Sponsor
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Collaborator
Citations
Deuterium and oxygen-18 measurements on microliter ...
Enriched levels of 2H (580%) and 18O (256%) in urine, plasma, saliva, and human milk can be measured with a precision of 3.2% (n = 200) and 0.97% (n = 200
2.
researchgate.net
researchgate.net/publication/42088578_Continuous_flow_2H1H_and_and_18O16O_analysis_of_water_samples_with_dual_inlet_precisionContinuous flow 2H/1H and and 18O/16O analysis of water ...
Total energy expenditure assessed by salivary doubly labelled water analysis and its relevance for short-term energy balance in humans.
Rapid measurements of water isotopes in human breath ...
We will also measure 2H:1H and 18O:16O enrichments in urine, saliva, and breath of 10 relatively healthy human subjects after consuming a standard DLW dose and ...
Rob BW 15-10d.indd - RePub, Erasmus University Repository
Energy expenditure and total body water outcomes of urine and saliva ... infant urine samples for 2H/1H and 18O/16O isotope ratio measurements.
Validation of deuterium and oxygen18 in urine and saliva ...
With LS, the precision is better for both 18O/ 16O and 2H/1H. New is the ability to measure 17O/16O with the same accuracy as 18O/16O. A ...
Exposure Factors Handbook - Chapter 6: Inhalation Rates
stable isotopes deuterium (2H) and heavy oxygen-18. (18O) are monitored in urine, saliva, or blood samples over a long period of time (from 7 ...
Validation of saliva sampling for total body water ... - PubMed
The H2 18O abundance in saliva reached a constant value by three hours after isotope administration, and the total body water calculated from the 3-h saliva ...
Introduction to body composition assessment
Deuterium is a stable (non-radioactive) isotope of hydrogen, with the symbol 2H. It is given orally as deuterium oxide (2H2O), and after mixing with body water ...
9.
medicaljournalssweden.se
medicaljournalssweden.se/actaodontologica/article/download/37249/42388/94861High-resolution H NMR investigations of the oxidative ...
High-field 1H NMR analysis provides much valuable molecular information regarding the fate of tooth- whitening oxidants in human saliva and permits an ...
10.
biometeorology.umn.edu
biometeorology.umn.edu/sites/biometeorology.umn.edu/files/2021-04/schultz_rcm.pdfIdentification and correction of spectral contamination in 2H ...
interfere with the measured 2H/1H and 18O/16O ratios.[10]. Therefore, the compounds most likely to cause contamination are methanol (MeOH) ...
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