90 Participants Needed

CAR T Cell Therapy for Pediatric Brain Cancer

NV
RR
Overseen ByRebecca Ronsley, MD
No Placebo GroupAll trial participants will receive the active study treatment (no placebo)

Trial Summary

What is the purpose of this trial?

This is a Phase 1 study of central nervous system (CNS) locoregional adoptive therapy with autologous CD4+ and CD8+ T cells lentivirally transduced to express a B7H3-specific chimeric antigen receptor (CAR) and EGFRt. CAR T cells are delivered via an indwelling catheter into the tumor resection cavity or ventricular system in children and young adults with diffuse intrinsic pontine glioma (DIPG), diffuse midline glioma (DMG), and recurrent or refractory CNS tumors. A child or young adult meeting all eligibility criteria, including having a CNS catheter placed into the tumor resection cavity or into their ventricular system, and meeting none of the exclusion criteria, will have their T cells collected. The T cells will then be bioengineered into a second-generation CAR T cell that targets B7H3-expressing tumor cells. Patients will be assigned to one of 3 treatment arms based on location or type of their tumor. Patients with supratentorial tumors will be assigned to Arm A, and will receive their treatment into the tumor cavity. Patients with either infratentorial or metastatic/leptomeningeal tumors will be assigned to Arm B, and will have their treatment delivered into the ventricular system. The first 3 patients enrolled onto the study must be at least 15 years of age and assigned to Arm A or Arm B. Patients with DIPG will be assigned to Arm C and have their treatment delivered into the ventricular system. The patient's newly engineered T cells will be administered via the indwelling catheter for two courses. In the first course patients in Arms A and B will receive a weekly dose of CAR T cells for three weeks, followed by a week off, an examination period, and then another course of weekly doses for three weeks. Patients in Arm C will receive a dose of CAR T cells every other week for 3 weeks, followed by a week off, an examination period, and then dosing every other week for 3 weeks. Following the two courses, patients in all Arms will undergo a series of studies including MRI to evaluate the effect of the CAR T cells and may have the opportunity to continue receiving additional courses of CAR T cells if the patient has not had adverse effects and if more of their T cells are available. The hypothesis is that an adequate amount of B7H3-specific CAR T cells can be manufactured to complete two courses of treatment with 3 or 2 doses given on a weekly schedule followed by one week off in each course. The other hypothesis is that B7H3-specific CAR T cells can safely be administered through an indwelling CNS catheter or delivered directly into the brain via indwelling catheter to allow the T cells to directly interact with the tumor cells for each patient enrolled on the study. Secondary aims of the study will include evaluating CAR T cell distribution with the cerebrospinal fluid (CSF), the extent to which CAR T cells egress or traffic into the peripheral circulation or blood stream, and, if tissues samples from multiple timepoints are available, also evaluate disease response to B7-H3 CAR T cell locoregional therapy.

Will I have to stop taking my current medications?

The trial requires that you stop taking certain medications before enrolling. You must discontinue chemotherapy, immunotherapy, and radiotherapy, and stabilize or reduce corticosteroid treatment to a specific dose before joining the trial.

What data supports the effectiveness of this treatment for pediatric brain cancer?

Research shows that CAR T cells targeting B7-H3, a protein found on many cancer cells, have shown strong activity against pediatric brain tumors in early studies. In particular, these cells have been effective in animal models of brain tumors, suggesting they could be a promising treatment option.12345

Is CAR T cell therapy safe for children with brain cancer?

CAR T cell therapy has shown some safety in early trials for pediatric brain cancer, with no dose-limiting toxicities reported. However, there were some serious side effects like headache and liver enzyme elevation, and one patient had a catheter-related infection not directly linked to the therapy.12678

How is the treatment SCRI-CARB7H3(s) different from other treatments for pediatric brain cancer?

SCRI-CARB7H3(s) is a unique treatment for pediatric brain cancer because it uses CAR T cells (a type of immune cell modified to attack cancer) that specifically target the B7-H3 protein, which is commonly found on these tumors. Unlike traditional treatments, it is administered directly into the brain, allowing for more effective targeting of the tumor with fewer side effects.123910

Research Team

RR

Rebecca Ronsley, MD

Principal Investigator

Seattle Children's Hospital

Eligibility Criteria

This trial is for children and young adults aged 1 to 26 with specific brain tumors like DIPG, DMG, or other recurrent CNS tumors without standard treatment options. They must be able to undergo apheresis (a procedure to collect T cells), have a catheter in their CNS for drug delivery, expect to live at least 8 weeks, have decent performance status scores (Lansky/Karnofsky ≥60), recovered from prior treatments, stable on low-dose steroids, good organ function and lab values.

Inclusion Criteria

I am between 1 and 26 years old.
My brain cancer has returned or is not responding to treatment, or I have DIPG/DMG after finishing standard therapy.
Your blood test results are within the normal range.
See 8 more

Exclusion Criteria

I am currently suffering from a severe infection.
I am currently on cancer treatment drugs.
Pregnant or breastfeeding
See 7 more

Timeline

Screening

Participants are screened for eligibility to participate in the trial

2-4 weeks

Treatment

Participants receive B7H3-specific CAR T cell therapy delivered via an indwelling catheter into the tumor resection cavity or ventricular system. Treatment involves two courses with weekly or bi-weekly dosing depending on the treatment arm.

8 weeks

Follow-up

Participants are monitored for safety and effectiveness after treatment, including MRI evaluations and assessment of CAR T cell distribution and disease response.

up to 7 months

Extension

Participants may continue receiving additional courses of CAR T cells if no adverse effects are observed and more T cells are available.

Treatment Details

Interventions

  • SCRI-CARB7H3(s)
Trial OverviewThe study tests B7-H3-specific CAR T cell therapy delivered directly into the brain or ventricular system through an indwelling catheter. It's divided into three arms based on tumor location/type. Patients receive two courses of treatment with weekly doses of engineered T cells targeting B7H3-expressing tumor cells. The goal is safe administration and interaction of these CAR T cells with the tumor.
Participant Groups
3Treatment groups
Experimental Treatment
Group I: ARM C (DIPG)Experimental Treatment1 Intervention
Patients with DIPG for whom CAR T cells will be delivered into the ventricular system
Group II: ARM B (Ventricular System Infusion)Experimental Treatment1 Intervention
Patients with non-DIPG either infratentorial tumors or leptomeningeal tumors for which the CAR T cells will be delivered into the ventricular system
Group III: ARM A (Tumor Cavity Infusion)Experimental Treatment1 Intervention
Patients with non-DIPG supratentorial tumors for which CAR T cells will be delivered into the tumor resection cavity

Find a Clinic Near You

Who Is Running the Clinical Trial?

Seattle Children's Hospital

Lead Sponsor

Trials
319
Recruited
5,232,000+

Findings from Research

CAR-T cell therapy, while currently approved only for B cell malignancies, shows promising potential for treating pediatric brain tumors, with early clinical trials indicating some positive responses.
Recent preclinical studies have identified new tumor antigens and combination strategies that enhance the effectiveness of CAR-T cells, particularly when administered directly to the tumor site.
CAR-T cells for pediatric brain tumors: Present and future.Leruste, A., Beccaria, K., Doz, F.[2021]
B7-H3 CAR T cells have shown significant antitumor activity in various pediatric solid tumor models, including osteosarcoma and medulloblastoma, indicating their potential as a treatment option for relapsed pediatric malignancies.
The effectiveness of B7-H3 CAR T cells is linked to the density of the B7-H3 antigen on tumor cells, suggesting that this therapy could be selectively effective against tumors with high antigen expression while minimizing effects on normal tissues with lower expression.
CAR T Cells Targeting B7-H3, a Pan-Cancer Antigen, Demonstrate Potent Preclinical Activity Against Pediatric Solid Tumors and Brain Tumors.Majzner, RG., Theruvath, JL., Nellan, A., et al.[2021]
Atypical teratoid/rhabdoid tumors (ATRTs) express the B7-H3 protein, which is crucial for their growth and can be targeted for therapy, highlighting its potential as a therapeutic target for this aggressive pediatric cancer.
Using B7-H3-targeted CAR T cells delivered directly into the brain showed faster and more effective tumor suppression in mouse models compared to traditional intravenous delivery, suggesting that localized treatment may reduce systemic side effects and improve outcomes for children with ATRT.
Locoregionally administered B7-H3-targeted CAR T cells for treatment of atypical teratoid/rhabdoid tumors.Theruvath, J., Sotillo, E., Mount, CW., et al.[2022]

References

CAR-T cells for pediatric brain tumors: Present and future. [2021]
CAR T Cells Targeting B7-H3, a Pan-Cancer Antigen, Demonstrate Potent Preclinical Activity Against Pediatric Solid Tumors and Brain Tumors. [2021]
Locoregionally administered B7-H3-targeted CAR T cells for treatment of atypical teratoid/rhabdoid tumors. [2022]
Advances in CAR T cell immunotherapy for paediatric brain tumours. [2022]
B7-H3 as a Novel CAR-T Therapeutic Target for Glioblastoma. [2020]
The Road to CAR T-Cell Therapies for Pediatric CNS Tumors: Obstacles and New Avenues. [2022]
Cross-study safety analysis of risk factors in CAR T cell clinical trials: An FDA database pilot project. [2022]
Expansion of endogenous T cells in CSF of pediatric CNS tumor patients undergoing locoregional delivery of IL13R〿2-targeting CAR T cells: an interim analysis. [2023]
Cell-surface antigen profiling of pediatric brain tumors: B7-H3 is consistently expressed and can be targeted via local or systemic CAR T-cell delivery. [2023]
10.United Statespubmed.ncbi.nlm.nih.gov
A novel anti-B7-H3 chimeric antigen receptor from a single-chain antibody library for immunotherapy of solid cancers. [2022]